In this research, the highly selective heterogeneous cation exchange membrane based on metal-organic frame works (MOFs) was prepared and introduced to electrodialysis system as hopeful material for the removal of heavy metal ions. MIL-101 (Fe) particles were fabricated via a simple chemical technique and suggested into matrix of the ion exchange membranes as additive particles. The membranes were prepared via solution casting technique by applying the desired concentrations of additive particles (0, 0.5, 1, 1.5, 2, 3 (wt%)) and then the effect of the additive particles on the morphology, electrochemical properties, and overall performance in electrodialysis system was studied. the synthesized MOF and homemade membranes were characterized using FTIR, FESEM, EDX and AFM. the images related to morphological studies showed uniform distribution of MIL-101 (Fe) particles in matrix of membranes. Utilizing MIL-101 (Fe) particles in the membrane body led to increase of surface hydrophilicity. The Water content of modified membranes was enhanced considerably compare to pristine. The membrane potential, transport number and permselectivity were increased by applying MIL-101 (Fe) loading ratio up to 1.5 wt% and then decreased. MIL-101 (Fe) membranes also, have considerable performance in removal of lead ions, so that modified sample with 2 wt% of MOF particles displayed a significant increase of 190% compare to pristine. The results are useful for electo-membrane process specially electrodialysis in order to water treatment.