آنالیز مکانیکی سلول‌های سالم و سرطانی استخوانی انسان با استفاده از میکروسکوپ نیروی اتمی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مکانیک، دانشکده فنی و مهندسی، دانشگاه آزاد اسلامی واحد دماوند، دماوند، تهران، ایران،

2 گروه علوم پایه، دانشکده فنی و مهندسی، دانشگاه آزاد اسلامی واحد دماوند، دماوند، تهران، ایران

چکیده
در این مطالعه، خواص مکانیکی سلول‌های بنیادی مزانشیمی (MSC) و سلول‌های سرطانی بافت استخوان (MG-63 و SAOS-2) با استفاده از میکروسکوپ نیروی اتمی (AFM) بررسی شده است. به دلیل شباهت‌های قابل‌توجه ویژگی‌های مکانیکی سلول‌های استئوبلاست انسانی نرمال (NHOst) و سلول‌های بنیادی مزانشیمی (MSC)، به‌جای NHOst از MSC استفاده شد. نتایج نشان داد که مدول الاستیسیته سلول‌های MG-63 و SAOS-2 نسبت به MSC کمتر است. مدول الاستیسیته این سلول‌ها، پیش و پس از درمان‌های شیمیایی و پلاسما اندازه‌گیری شد. برای تعیین دوزهای مناسب برای انکوبه‌سازی 24 و 48 ساعته، از سنجش MTT استفاده شد تا غلظت IC50 سلول‌ها مشخص گردد. نتایج نشان داد که مدول‌های الاستیک سلول‌های MG-63 (917 پاسکال) به میزان 1.72 (1579 پاسکال) و 5.44 برابر (4985 پاسکال) (پس از 24 و 48 ساعت) نسبت به سلول‌های MG-63 درمان‌نشده و مدول‌های الاستیک سلول‌های SAOS-2 (697 پاسکال) به میزان 1.15 (802 پاسکال) و 7.49 برابر (5225 پاسکال) (24 و 48 ساعت) نسبت به سلول‌های SAOS-2 درمان‌نشده افزایش پیدا کرده‌اند. درمان پلاسما باعث افزایش مدول‌های الاستیک سلول‌های MG-63 و SAOS-2 شد. در بخش بعدی، فرکانس‌های رزونانس و تغییرات تابع پاسخ فرکانسی حرکت‌های تیر AFM با استفاده از شبیه‌سازی‌های عددی (FEM) و آزمایش‌های تجربی موردبررسی قرار گرفت. نتایج نشان داد که افزایش سختی نمونه‌ها، فرکانس رزونانس را بالا می‌برد. درنهایت، نتایج عددی و تجربی با یکدیگر مقایسه شدند و توافق خوبی بین آن‌ها مشاهده شد.

کلیدواژه‌ها


عنوان مقاله English

Mechanical Analysis of Human Healthy and Cancerous Bone Cells Applying Atomic Force Microscope

نویسندگان English

Ali Jafari 1
Ali Sadeghi 1
Mansoureh Lafouti 2
1 Department of Mechanical Engineering, Damavand branch, Islamic Azad University, Damavand, Tehran, Iran
2 Department of Basic Sciences, Damavand branch, Islamic Azad University, Tehran, Iran
چکیده English

In the present investigation, the mechanical properties of mesenchymal stem cells (MSC) and carcinomatous cells of bone tissue (MG-63 and SAOS-2) has been studied applying AFM. Based on the sufficient similarity of mechanical characterizations of normal human osteoblast cells (NHOst) with mesenchymal stem cells (MSC), MSC have been applied instead to NHOst. Due to the outcomes, the elastic modules of MG-63 and SAOS-2 are lower than MSC. The elastic modulus of MG-63 and SOAS-2 cells were estimated before and after chemo and plasma treatment. MTT appraisal has been applied to define the convenient dosages for 24- and 48-h incubations due to the IC50 cell viability concentration. The elastic modules of MG-63 (917 Pa) and SAOS-2 (697 Pa) cell increase to 1.72 (1579 Pa) and 5.44 (4985 Pa) (after 24, 48 h) times compared to untreated MG-63 cell and 1.15 (802 Pa) and 7.49 (5225 Pa) (24, 48 h) times compared to untreated SAOS-2 cell. The plasma treatment increased the elastic modules of MG-63 and SAOS-2 cells. In the second section, the resonant frequencies and enlargement of the frequency response function of the AFM beam’s motions have been analyzed using FEM and experimental procedures by AFM. The outcomes displayed that raising the specimens’ hardness raises the resonant frequency. Lastly, the FEM and experimental outcomes have been evaluated and displayed the good agreement.

کلیدواژه‌ها English

Young’s modulus
Carcinoma cell
Chemo treated
Plasma treated
Atomic force microscope
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دوره 4، شماره 3
پاییز 1403
صفحه 513-540

  • تاریخ دریافت 12 بهمن 1403
  • تاریخ بازنگری 06 اسفند 1403
  • تاریخ پذیرش 12 اسفند 1403