Mechanic of Advanced and Smart Materials
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Mechanical Analysis of Human Healthy and Cancerous Bone Cells Applying Atomic Force Microscope

Document Type : Original Article

Authors

Ali Jafari 1
Ali Sadeghi 1
Mansoureh Lafouti 2

1 Department of Mechanical Engineering, Damavand branch, Islamic Azad University, Damavand, Tehran, Iran

2 Department of Basic Sciences, Damavand branch, Islamic Azad University, Tehran, Iran

10.61186/masm.4.3.513
Abstract
In the present investigation, the mechanical properties of mesenchymal stem cells (MSC) and carcinomatous cells of bone tissue (MG-63 and SAOS-2) has been studied applying AFM. Based on the sufficient similarity of mechanical characterizations of normal human osteoblast cells (NHOst) with mesenchymal stem cells (MSC), MSC have been applied instead to NHOst. Due to the outcomes, the elastic modules of MG-63 and SAOS-2 are lower than MSC. The elastic modulus of MG-63 and SOAS-2 cells were estimated before and after chemo and plasma treatment. MTT appraisal has been applied to define the convenient dosages for 24- and 48-h incubations due to the IC50 cell viability concentration. The elastic modules of MG-63 (917 Pa) and SAOS-2 (697 Pa) cell increase to 1.72 (1579 Pa) and 5.44 (4985 Pa) (after 24, 48 h) times compared to untreated MG-63 cell and 1.15 (802 Pa) and 7.49 (5225 Pa) (24, 48 h) times compared to untreated SAOS-2 cell. The plasma treatment increased the elastic modules of MG-63 and SAOS-2 cells. In the second section, the resonant frequencies and enlargement of the frequency response function of the AFM beam’s motions have been analyzed using FEM and experimental procedures by AFM. The outcomes displayed that raising the specimens’ hardness raises the resonant frequency. Lastly, the FEM and experimental outcomes have been evaluated and displayed the good agreement.

Keywords

Young’s modulus
Carcinoma cell
Chemo treated
Plasma treated
Atomic force microscope
Mechanic of Advanced and Smart Materials
Volume 4, Issue 3
Autumn 2024
Pages 513-540

  • Receive Date 31 January 2025
  • Revise Date 24 February 2025
  • Accept Date 02 March 2025

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