Mechanic of Advanced and Smart Materials
Quarterly

Defining of Mechanical Properties of Ago-1522 Living Cell Using Atomic Force Microscope

Document Type : Original Article

Authors

Ali Sadeghi 1
Adel Pour Esmaeil Fatideh 1
Matineh Mohseni 2
Fatemeh Shahrizadegan 2

1 Department of Mechanical Engineering, Damavand branch, Islamic Azad University, Damavand, Tehran, Iran

2 Department of Biomechanical Engineering, Central Tehran branch, Islamic Azad University, Tehran, Iran

https://doi.org/10.52547/masm.2.1.1
Abstract
In general, every normal cell of the human body, which consists of different parts such as the nucleus, cell membrane and protein nanofibers, has a certain mechanical hardness and strength. Any noticeable change in this strength indicates a disease. One way to diagnose various diseases such as cancer in normal cells of the human body is to measure the amount of changes that have occurred in their mechanical properties. In the present paper, mechanical properties including modulus of elasticity and adhesion force of Ago-1522 cell, which is considered as a natural skin cell, using JPK atomic force microscope (made in Germany), Nano Wizard 3 in both extension and retraction modes, and obtained at a temperature of 37 degrees Celsius. Based on the results, the average elastic modulus for the cell in the extension mode (as the valid mode in the reports) is equal to 574.4 Pa. The adhesion force is equivalent to N in retraction mode. Based on images taken from the surface of the cell with an atomic force microscope, its maximum height is 0.4 micrometers.

Keywords

Atomic force microscope
Elasticity modulus
Adhesion force
Living cell
Mechanical properties
[1] Binnig G, Quate C F, Gerber C. Atomic force microscope. Physical review letters. 1986;56:930-934.
[2] Hanahan D, Weinberg R A. Hallmarks of cancer: the next generation. cell. 2011;144:646-674.
[3] Jinka R, Kapoor R, Sistla P G, Raj T A, Pande G. Alterations in cell-extracellular matrix interactions during progression of cancers. International journal of cell biology. 2012;2012.
[4] Lekka M. Discrimination between normal and cancerous cells using AFM. Bionanoscience. 2016;6:65-80.
[5] Lekka M, Laidler P, Gil D, Lekki J, Stachura Z, Hrynkiewicz A Z. Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy. European Biophysics Journal. 1999;28:312-316.
[6] Au N P B, Fang Y, Xi N, Lai K W C, Ma C H E. Probing for chemotherapy-induced peripheral neuropathy in live dorsal root ganglion neurons with atomic force microscopy. Nanomedicine: Nanotechnology, Biology and Medicine. 2014;10:1323-1333.
[7] Hung M-S, Tsai M-F. Investigating the influence of anti-cancer drugs on the mechanics of cells using AFM. Bionanoscience. 2015;5:156-161.
[8] Li M, Liu L-q, Xi N, Wang Y-c. Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy. Acta Pharmacologica Sinica. 2015;36:769-782.
[9] Adami B S, Diz F M, Gonçalves G P O, Reghelin C K, Scherer M, Dutra A P, Papaléo R M, de Oliveira J R, Morrone F B, Wieck A. Morphological and mechanical changes induced by quercetin in human T24 bladder cancer cells. Micron. 2021;151:103-152.
Mechanic of Advanced and Smart Materials
Volume 2, Issue 1
Spring 2022
Pages 1-12

  • Receive Date 12 April 2022
  • Revise Date 04 May 2022
  • Accept Date 15 May 2022

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